Raj Kandpal, PhD
Professor of Biochemistry
Assistant Chair of BMS
College of Osteopathic Medicine of the Pacific
Join year: August 2007
Ph.D., Biochemistry, Indian Institute of Science, Bangalore, India, 1985
M.Sc., Biochemistry, G.B. Pant University, Pantnagar, India, 1977
B.Sc., Physics/Chem/Math, Kumaon University, Nainital, India, 1974
Postdoctoral Research Fellow, Molecular Biology Institute, UCLA, Los Angeles, CA, 1985
Associate Professor, College of Osteopathic Medicine of the Pacific, Pomona, CA, 2007-Present
Associate Professor, Department of Biological Sciences, Fordham University, Bronx, NY, 2000-2007
Assistant Professor, Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, PA, 1993-2000
Molecular and Cellular Basis of Medicine (Molecular Biology, Cell Biology and Genetics)
Molecular and Cellular Basis of Life (Molecular Biology, Cell Biology and Genetics)
Endocrine System (Cholesterol Metabolism, Bile Acids and Salts, Vitamin D and Steroid Hormones)
Cardiovascular System (Cardiac Metabolism; Atherosclerosis and Genetics of Cardiovascular Diseases)
We have been using cell biological, molecular biological and genetic approaches to understand mechanisms underlying disease processes and indentifying targets for therapeutic interventions. We have been applying these approaches toward investigating breast, prostate and ovarian cancer, diabetic retinopathy, and genetic deafness. The following is a brief description of some projects currently underway or proposed projects in my laboratory.
- Epigenetic mechanisms of breast cancer invasiveness: We have profiled the expression of Eph receptors, the largest family of receptor tyrosine kinases, and their ephrin ligands in breast carcinoma cells. We are currently exploring epigenetic mechanisms responsible for the loss of EphB6 receptor in invasive breast carcinoma cells, and characterizing interactions among Eph receptors, and between Eph receptors and cadherins. In addition, we are developing methylation sensitive PCR (MSP) assay of EphB6 promoter as a diagnostic tool to determine estrogen-positive and estrogen-negative cells in breast tumors. Another aspect of our research is aimed at determining the transcriptomic profiles of cancer stem cells isolated from breast carcinoma cells of varying degress of invasivness.
- Molecular basis of diabetic retinopathy: In collaboration with investigators in the Neurobiology, Neurodegeneration and Repair Laboratory (NNRL) at the National Eye Institute, we have recently embarked on investigating the mRNA, miRNA, proteomic and mitochondrial bioenergietic profiles of normal and diabetic retina. The objective of these studies is to unravel the molecular consequences of diabetes in photoreceptor cells in the retina.
- Candidate genes involved in human deafness: Human deafness, genetic or acquired, is a major public health concern. A large number of nonsyndromic hearing loss phenotypes have been mapped to various loci on human chromosomes. We have used in silico approaches to catalog a list of candidate genes from chromosome regions linked to deafness phenotypes. We propose to characterize selected candidate cDNAs for their potential involvement in inner ear function.
- Kandpal RP, Sandhu AK, Kaur G, Kaur GP and Athwal RS (2017). Monochromosomal hybrids and chromosome transfer: A Funtional approach for gene identification. Cancer Genomics Proteomics 14, 93-102.
- Johnson C, Segovia B, Kandpal RP. EPHA7 and EPHA10 Physically Interact and Differentially Co-localize in Normal Breast and Breast Carcinoma Cell Lines, and the Co-localization Pattern is Altered in EPHB6-expressing MDA-MB-231 Cells. Cancer Genomics Proteomics. 2016 09-10:13(5):359-68. PMID 27566654
- Mishra B, Swaroop A and Kandpal RP. (2016) Genetic components in diabetic retinopathy. Indian J Ophtlmol. 64, 55-61. March 2016 doi: 10.4103/0301-4738.178153.
- Bhushan L, Tavitian N, Dey D, Tumur Z, Parsa C, Kandpal RP (2014). Modulation of Liver-Intestine Cadherin (Cadherin 17) Expression, ERK Phosphorylation and WNT Signaling in EPHB6 Receptor-expressing MDA-MB-231 Cells. Cancer Genomics Proteomics. 09-10;11(5):239-249
- Kandpal RP, Rajasimha HK, Brooks MJ, Nellissery J, Wan J, Qian J, Kern TS and Swaroop A (2012). Transcriptome analysis using next generation sequencing reveals molecular signatures of diabetic retinopathy and efficacy of candidate drugs. Mol Vis 18: 1123-1146.
- Rane NS, Sandhu AK, Zhawar VS, Kaur G, Popescu NC, Kandpal RP*, Jhanwar-Uniyal M, Athwal RS*, (2011) Restoration of Senescence in Breast and Ovarian Cancer Cells Following the Transfer of the YAC Carrying SEN6A Gene Located at 6q16.3 Cancer Genomics Proteomics 8, 227-234. (*Co-corresponding authors)
- Bhushan L and Kandpal RP (2012). EphB6 receptor. Atlas Genet Cytogenet Oncol Hematol.February 2012. http://AtlasGeneticsOncology.org/Genes/EPHB6ID40471ch7q34.html
- Bhushan, L. and Kandpal, RP (2011), EphB6 Receptor Modulates Micro RNA Profile of Breast Carcinoma Cells. PLoS One, 6(7): e22484.doi:10.1371/journal.pone.0022484.
- Fox, BP and Kandpal, RP. (2011), A Paradigm Shift in Eph Receptor Interaction: Biological Relevance of EphB6 Interaction with EphA2 and EphB2 in Breast Carcinoma Cell Lines. Cancer Genomics Proteomics, 8, 185-193.
- Kandpal, RP. (2010), Tyrosine Kinase-Deficient EphB6 Receptor-Dependent Alterations in Proteomic Profiles of Invasive Breast Carcinoma Cells as Determined by Difference Gel Electrophoresis. Cancer Genomics Proteomics, 7, 253-260.
- Zhawar, VK, Kaur, G, deRiel, JK, Kaur, GP, Kandpal, RP and Athwal, RS (2010), Novel spliced variants of ionotropic glutamate receptor GluR6 in normal human fibroblast and brain cells are transcribed by tissue specific promoters. Gene. 459, 1-10
- Fox, BP and Kandpal RP. (2010) DNA-based assay for EPHB6 expression in breast carcinoma cells as potential diagnostic test for detecting tumor cells in circulation. Cancer Genomics Proteomics, 7, 9-16.
- Liu J, Kaur G, Zhawar VK, Zimonjic DB, Popescu NC, Kandpal RP, Athwal RS. (2009) Role of SV40 integration site at chromosomal interval 1q21.1 in immortalized CRL2504 cells. Cancer Res. 69:7819-25.
- Kandpal, R.P., Saviola, B. and Felton, J. (2009) The era of ’Omics unlimited. Biotechniques. 46, 351-355.
- Liu, J., Kaur, G., Kaur, G.P., deRiel, J.K., Kandpal, R.P. and Athwal, R.S. (2009) Chromosome 6 encoded cytoplasmic RNaseT2 protein is a cell growth regulator. J. Cell. Mol. Med. 14, 1146-1155.
- Fox, B.P. and Kandpal, R.P. (2009) EphB6 Receptor Significantly Alters Invasiveness and Other Phenotypic Characteristics of Human Breast Carcinoma Cells. Oncogene. 28, 1706–1713.
- Fox, B.P. and Kandpal R.P. (2008) Yeast two-hybrid assay reveals several interactors of EphB6 receptor. The Open Proteomics J. 1, 79-86.
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