Background

Awards and Honors:

2009 American Heart Association Peer Reviewer (Microbiology/Virology study section)
2009 American Osteopathic Association Research Grant Reviewer
2008 Outstanding Research Presentation Award COM-2008, UIC
2007 The Glaucoma Foundation Research Grant Co-Investigator Award
2007 Research Award & Certificate of Recognition, Illinois Society to Prevent Blindness
2006 Research Award & Certificate of Recognition, Illinois Society to Prevent Blindness
2006 Mid West Eye Bank Research Grant Co-Investigator Award
2006 Board of Trustee of University of Illinois at Chicago Service Award
2006 Travel Award for International Herpes Workshop (Seattle)
2005 Research Award & Certificate of Recognition, Illinois Society to Prevent Blindness
2005 American Heart Association Postdoctoral Fellowship (2005-2007)
1997 Senior Research Fellow Award, University Grant Commission, India
1994 Junior Research Fellow Award, University Grant Commission, India
1994 Graduate Aptitude Test in Engineering: Indian Institute of Technology (IIT), India
1992 First Division & First Rank Certificate in MS
1990 First Division & Second Rank in BS
1987 First Division U.P. Board Intermediate Examination, India
1985 First Division U.P. Board High School Examination, India

International Patents:

2002 Purified and isolated heparan sulfate 3-O-sulfotransferase isoform 5 nucleic acids and polypeptides and therapeutic and screening methods. UIC Reference Number CW071. US Provisional Patent Application Serial No. 60/394, 199.

2004 Anti-HSV activity of a plant extract. University of Illinois at Chicago Invention Disclosure Application Number CY013 filled for U.S. Patent at UIC office.

2005 Anti-HSV activity of a of lignin sulfate. A joint patent pending with Virginia Commonwealth University: Invention Disclosure Applicationnumber CY032 submitted for U.S. Patent at UIC Office of Technology Management.

2006 Isolation and characterization of 12-mer peptides generated against Heparan Sulfate. Invention Disclosure Applicationnumber (Application Number DA01015PPA) submitted for U.S. Patent at UIC Office of Technology Management. Attorney Docket No. 7940-7.

2006 Construction of 12-mer peptides library generated against 3-O-sulfated heparan sulfate modified by 3-O-sulfotransferase-3 (3-OST-3). Application submitted for U.S. Patent at UIC office of Technology Management.

2006 Cover page of “Virology” (Elsevier Science) Journal (2006; March 15th issue; Volume 346: 452-459) selected the picture of the cell-fusion mediated by 3-O-sulfotransferase isoform-2 in assisting HSV-1 spread (O Donnell & Tiwari et al., 2006).

2007 Research news in eyePRO (UIC) for Glaucoma Foundation Grant Award for research on Herpes Simplex-induced glaucoma by Dr. Shukla and Dr. Tiwari.

2008 Novel Role of HSV-1 gB on LARS formation and Extra cellular Viral Transport submitted for U.S. Patent at UIC office of Technology Management.

2008 First Prize Winner Certificate Award (Cash Prize of $ 500 and $ 1500 travel award) at College of Medicine (COM-2008) Outstanding Research Presentation at University of Illinois at Chicago (UIC) by Dr. Larry Tobacman. (21st February 2008).

Membership:

Professional Member of American Heart Association (AHA)
Member of Council on Arteriosclerosis, Thrombosis and Vascular Biology
International Atherosclerotic Society
American Association for Advancement for Science (AAAS)
American Society for Microbiology (ASM)
Association for Research in Vision and Ophthalmology (ARVO)
American Association of Virologist (ASV)
International Society for Antiviral Research (ISAR)
Chicago Area Virologist Association (CAVA)

Research Interests:

Molecular Mechanism of Herpes simplex virus (HSV) Entry into a cell
HSV-1 infection and Heart Diseases
HSV-1entry and spread in Neurons
HSV-induced Ocular infection and Stromal Keratitis
Herpes viruses Associated Infections
Cell Biology of Viral Infections
Development of Synthetic Peptides; Pharmacological Agents against Herpes Viruses Infection
Development of Novel Anti-HSV therapies from Natural Resources & Synthetic origin
Antiviral from Medicinal Plants

Research Support:

7/1/2007-6/30/2008 The Glaucoma Foundation Research Grant Award. Novel peptides to understand herpetic damage to human trabecular meshwork via actin rich nanotubular structures.
Role: Co-Principal Investigator
Total Costs: $ 40,000.00

6/1/2007-5/31/2008 Illinois Society for the Prevention of Blindness. Use of Novel Peptides to Study Viral Spread in Human Ocular Cells.
Role: Principal Investigator
Total Costs: $ 5000.00

7/1/2005-6/30/2007 American Heart Association Greater Midwest Postdoctoral Award 0525768Z (Tiwari, V) Funding Agency: American Heart Association. The research project conducted for AHA ward is entitled ‘Structure-Function Aspects of Anticoagulation Heperan Sulfate.”
Role: Principal Investigator
Total Costs: $104, 040.00 (direct), $104, 040.00 (total)

6/1/2006-5/31/2007 Illinois Society for the Prevention of Blindness. Identifying the Receptors Crucial to Herpetic Damage to the Human Corneal Fibroblasts.
Role: Principal Investigator
Total Costs: $ 5000.00

7/1/2006-6/30/2007 Midwest Eye Bank Award. The research project being conducted by this award is entitled “Nectin-1 expression in Human Ocular Cells”.
Role: Co-Principal Investigator
Total Costs: $16,000.00

6/1/2005-5/31/2006 Illinois Society for the Prevention of Blindness. Identifying the Receptors Crucial to Herpetic Damage to the Human Trabecular Meshwork.
Role: Co- Principal Investigator
Total Costs: $ 4000.00

Peer Reviewed Publications:

1. Tiwari V., Darmani, N.A., Thrush, G., Shukla D (2009). An unusual dependence for heparan sulfate in Kaposi s sarcoma virus glycoprotein induced cell to cell fusion. Biochemical and Biophysical Research Communications (In Press). Manuscript # BBRC-09-5417
2. Tiwari, V., Shukla, S., Shukla D (2009). A sugar binding protein Cyanovirin-N blocks herpes simplex virus entry into cell fusion. Antiviral Research 84: 67-75. Manuscript # AVR-D-09-00104.
3. Kovacs, S.K., Tiwari, V., Prandovszky, E., Dosa, S., Bacsa, S., Valyi-Nagy, K., Shukla, D., Valyi-Nagy T (2009). Expression of Herpesvirus Entry Mediator (HVEM) in the Cornea and Trigeminal Ganglia of Normal and HSV-1 Infected Mice. Current Eye Research. 34(10):896-904.
4. Tiwari V., Oh MJ, Kovacs M, Shukla D (2008). Role for Nectin-1 in herpes simplex virus 1 entry and spread into Retinal Pigment Epithelial Cells. FEBS Journal 275: 5272-5285.
5. Dixit R*, Tiwari V*, Shukla D (2008). Herpes simplex virus type 1 induces filopodia in differentiated P19 neural cells to facilitate viral spread. Neuroscience Letter 440: 113-118.* Equally Contributed Authors.
6. Akhtar J, Tiwari V, Oh MJ, Kovacs M, Jani A, Kovacs SK, Valyi-Nagy T, Shukla D (2008). HVEM and nectin-1 determine herpes simplex virus (HSV-1) entry into human conjunctival epithelium. Investigative Ophthalmology Visual Sciences 49: 4026-4035.
7. Copland R, Balasubramaniam A, Tiwari V, Zhang F, Bridges A, Linhardt R, Shukla D, Liu J (2008). Using a 3-O-sulfated heparin octasaccharide to inhibit the entry of herpes simplex virus-1. Biochemistry 47: 5774-5783.
8. Tiwari V, G. B. ten Dam, Beatrice Y.J.T. Yue, T. H. van Kuppevelt, Shukla D (2007). Requirements of 3-O-sulfated heapran sulfate during cell fusion in primary cultures of human corneal fibroblasts. FEBS Letters 581: 4468-4472.
9. Tiwari V, Christopher O Donnell, Shukla D (2007). Soluble 3-OST-3 mediated HSV-1 entry into resistant CHO-K1 cells. Journal of General Virology 88: 1075-1079.
10. Tiwari V, S Shukla, Shukla D (2007). HSV-2 entry into cultured human corneal fibroblasts is mediated by herpes entry mediator. Journal of General Virology 88: 1075-1079.
11. Raghuraman A., Tiwari V, Zhao Q., Shukla D., Debnath AK, Desai U (2007). Viral Inhibition Studies on Sulfated Lignin, a Chemically Modified Biopolymer and a Potential Mimic of Heparan Sulfate. Biomacromolecules 8(5):1759-1763.
12. Kavouras J.H., Prandovszky, E., Valyi-Nagy, K., Kovacs, S.K., Tiwari, V., Kovacs, M., Shukla, D., Valyi-Nagy, T. (2007). Herpes simplex virus type-1 infection induces oxidative stress and the release of bioactive lipid per oxidation by-products in mouse P19N neural cell cultures. Journal of NeuroVirology 13: 1-10.
13. Clement C*, Tiwari V*, Scanlon P, Tibor Vali-Nagy, Yue BYJT, Shukla D (2006). A novel for phagocytosis-like uptake in herpes simplex virus entry. Journal of Cell Biology.174:1009-1021. *Equally Contributed Authors.
14. Tiwari, V., Clement, C., Xu, D., Valyi-Nagy, T., Yue, B.Y.J.T., Liu, J. & Shukla, D. (2006). Role for 3-O-sulfated heparan sulfate as a receptor for herpes simplex virus type-1 entry into primary human corneal fibroblasts. Journal of Virology 80:8970-8980.
15. Christopher O Donnell*, Tiwari V*, Myung Jin-Oh, Shukla D (2006). A Role for 3-O-sulfotransferase isoform-2 in assisting HSV-1 entry and spread. Virology 346: 452-459. *Equally Contributed Authors.
16. Tiwari V, Clement C, Scanlan PM, Yue BYJT, Shukla D (2005). A Role for HVEM as the Receptor for Herpes Simplex Virus-1 Entry into Primary Human Trabecular Meshwork Cells. Journal of Virology 79 (20): 13173-13179.
17. Tiwari V, Christopher O Donnell, Myung Jin-Oh, Tibor Valyi-Nagy, Shukla D (2005). A Role for 3-O-sulfotransferase isoform-4 in assisting HSV-1 entry and spread. Biochemical and Biophysical Research Communications 338: 930-937.
18. Xu D, Tiwari V, Guoqing Xia, D. Shukla, J. Liu (2005). Characterization of Heparan Sulfate 3-O-sulfotransferase Isoform 6 and its Role in Assisting the Entry of Herpes Simplex Virus Type- 1. Biochemical Journal 385: 451-459.
19. Raghuraman, A., Tiwari, V, Thakkar, J.N., Gunnarsson, T., Shukla, D., Hindle, M., Desai, U (2005). Structural characterization of a serendipitously discovered bioactive macromolecule, Lignin sulfate. Biomacromolecules 6 (5): 2822-2832.
20. Scanlan P, Tiwari V, Bommireddy S, Shukla D (2005). Spinoculation of heparan sulfate deficient CHO 745 cells enhances herpes simplex type-1 entry, but does not abolish the need for essential glycoproteins involved in the virus fusion mechanism. Journal of Virological Method 128 (1-2):104-112.
21. Shukla D, Scanlan P, Tiwari V, Sheth V, Clement C, Kavouras JH, Lu Leach, Grace Guzman-Hartman, Dermody TS, Tibor Valyi-Nagy (2005). Effect of Herpes Simplex Virus Type 1 Infection on Nectin-1 Expression in the Murine Brain. Applied Immunohistochemistry and Molecular Morphology 14(3):341-347.
22. Tiwari V, Clement C, Duncan MB, Chen J, Liu J, Shukla D (2004). The role for 3-O-sulfated heparan sulfate in cell-to-cell fusion mediated by herpes simplex virus type-1. Journal of General Virology, Society for General Microbiology 85: 805-809.
23. Tibor Valyi-nagy, Veeral Seth, Christian Clement, Tiwari V, Perry Scanlan, Jerry H. Kavouras, Lu Leach, Grace-Guzman-Hartman, Terence S. Dermody, Shukla D (2004). Herpes Simplex virus entry receptor nectin-1 is widely expressed in the human eye. Current Eye Research 29: 303-309.
24. Scanlan P, Tiwari V, Bommireddy S, Shukla D (2003). Cellular expression of gH confers resistance to Herpes Simplex Virus. Virology 312 (1): 14-24.
25. Xia G, Chen J, Tiwari V, Ju W, Jin-Ping Li, Malmström A, Shukla D, Liu J (2002). Heparan Sulfate 3-O-Sulfotransferase Isoform 5 Generates Both an Antithrombin-binding Site and an Entry Receptor for Herpes Simplex Virus Type 1. Journal of Biological Chemistry, 227(40): 37912-37919.