Yijia Xiong,

Ph.D. Physical Chemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China 1994-2000
B.S. Chemical Physics, University of Science and Technology of China, Hefei, China 1989-1994

Research Scientist, Pacific Northwest National Laboratory, Richland, Washington, 2010-2014
Postdoctoral Associate, Pacific Northwest National Laboratory, Richland, Washington, 2002-2010
Postdoctoral Associate, Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas, 2001-2002

My research interest focus in the development and application of advanced fluorescence technology, including fluorescence correlation spectroscopy, time-resolved frequency domain fluorescence anisotropy measurement, single molecule imaging etc., to study protein structure dynamic and protein-protein or protein-DNA interactions. We have developed a few bi-Arsenic fluorescent probes for specific labeling of target protein as well. Currently we have two research projects which focus on the stabilization of protein structures and activities in confined materials, e.g. protein based smart hydrogel or diatom based biosilica. We have entrapped proteins in a protein based smart hydrogel and shown improved stability in chaotic reagents. We can also express protein in diatom to incorporate into biosilica. When specific probes, e.g. single chain antibody, were entrapped, the material can also be used for highly sensitive fluorescence detection of pathogen or threat agents.

Genetically-Engineered, Nanoscale, Biosilica-Immobilized Functional Materials. Defense Threat Reduction Agency. 2012-2015
Role of Confinement and Material Surface on Protein Dynamics and Function. Defense Threat Reduction Agency. 2011-2016

Outstanding Performance Award, Fundamental and Computational Science Directorate, Pacific Northwest National Laboratory, 2010
Scholarship for Outstanding Young Scientist, Institute of Chemistry, 1999

Biophysics Society
Protein Society

Selected Publications

• Beech, B. M., Xiong, Y., Boschek, C. B., Baird, C. L., Bigelow, D. J., McAteer, K., and Squier, T. C. (2014) Controlled Activation of Protein Rotational Dynamics Using Smart Hydrogel Tethering, Journal of the American Chemical Society 136, 13134-13137.
• Weber, T., Magnaldo, T., and Xiong, Y. (2014) ALDH1A1 Deficiency in Gorlin Syndrome Suggests a Central Role for Retinoic Acid and ATM Deficits in Radiation Carcinogenesis, Proteomes 2, 451-467.
• Fu, N.; Xiong, Y.; Squier, T. C., Optimized Design and Synthesis of a Cell-Permeable Biarsenical Cyanine Probe for Imaging Tagged Cytosolic Bacterial Proteins. Bioconjugate Chemistry 2013, 24 (2), 251-259.
• Fu, N.; Su, D.; Cort, J. R.; Chen, B.; Xiong, Y.; Qian, W.-J.; Konopka, A. E.; Bigelow, D. J.; Squier, T. C., Synthesis and Application of an Environmentally Insensitive Cy3-Based Arsenical Fluorescent Probe To Identify Adaptive Microbial Responses Involving Proximal Dithiol Oxidation. Journal of the American Chemical Society 2013, 135 (9), 3567-3575.
• Fu, N.; Xiong, Y.; Squier, T. C., Synthesis of a Targeted Biarsenical Cy3-Cy5 Affinity Probe for Super-resolution Fluorescence Imaging. Journal of the American Chemical Society 2012, 134 (45), 18530-18533.
• Smith, D. M. A.; Xiong, Y.; Straatsma, T. P.; Rosso, K. M.; Squier, T. C., Force-Field Development and Molecular Dynamics of [NiFe] Hydrogenase. Journal of Chemical Theory and Computation 2012, 8 (6), 2103-2114.
• Xiong, Y.; Chen, B.; Shi, L.; Fredrickson, J. K.; Bigelow, D. J.; Squier, T. C., Targeted Protein Degradation of Outer Membrane Decaheme Cytochrome MtrC Metal Reductase in Shewanella oneidensis MR-1 Measured Using Biarsenical Probe CrAsH-EDT2. Biochemistry 2011, 50 (45), 9738-9751.
• Cao, H.; Xiong, Y.; Wang, T.; Chen, B.; Squier, T. C.; Mayer, M. U., A Red Cy3-Based Biarsenical Fluorescent Probe Targeted to a Complementary Binding Peptide. J. Am. Chem. Soc., 2007, 129, 28, 8672 - 8673,
• Xiong, Y.; Chen, B.; Smallwood, H. S.; BieberUrbauer, R. J.; Markille, L. M.; Galeva, N.; Williams, T. D.; Squier, T. C., High-Affinity and Cooperative Binding of Oxidized Calmodulin by Methionine Sulfoxide Reductase. Biochemistry 2006, 45, (49), 14642-14654.
• Xiong, Y.; Shi, L.; Chen, B.; Mayer, M. U.; Lower, B. H.; Londer, Y.; Bose, S.; Hochella, M. F.; Fredrickson, J. K.; Squier, T. C., High-Affinity Binding and Direct Electron Transfer to Solid Metals by the Shewanella oneidensis MR-1 Outer Membrane c-type Cytochrome OmcA. Journal of the American Chemical Society 2006, 128, (43), 13978-13979.

Click link for complete list of publications: http://scholar.google.com/citations?user=JbrDaPAAAAAJ&hl=en&cstart=0&pagesize=20