Description of Research Facilities
The laboratory spaces allocated to the College of Veterinary Medicine (CVM) are located in three buildings within walking distance of each on the campus at Western University of Health Sciences in Pomona, California: the Health Education Center (HEC), Banfield Veterinary Clinic Center (BVCC) on the east side, and the Rodney P. Wineberg Center, formerly the University Research Center, (RWC/URC) on the west side of the campus.
Research space in the Health Education Center, Western University of Health Sciences, Pomona:
The CVM research space in HEC comprises two newly remodeled 235 and 130 sq. ft cell-culture rooms and 400 sq. ft of research space dedicated to non-infectious diseases and the isolation and culture of stem-cells. The clean rooms and research labs are equipped with four CO2 and O2 humidified incubators (New Brunswick), three biosafety cabinets (Logica, LabCONCO), two table top refrigerated centrifuges (Eppendrof Model 5810R, C-2500-R-Prism), a 5L analog water bath, an analytical balance, a shaking incubator (Excella E 24/ New Brunswick), one table top non-refrigerated centrifuge, two liquid nitrogen tanks dedicated to stem cell banking, a freeze-dry vacuum & pump (FreeZoon, 2.5, LabCONCO), a neon transfection system (Life Technology), a NanoDrop Lite (Thermo Fisher), a thermal cycler (Eppendorf), a QPCR (CFX- BioRad), a flow cytometer (BD Accuri, C6), a ELISA Machine (CAMBREX), a pH meter (Fisher Scientific), an oven (HERATHERM, Thermo Scientific), a vacuum aspirator collection system, a 38CF glass refrigerator, three combo Refridge-Freezer (Kenmore), two liquid N2 tanks (Thermo Scientific), one -20°C (Sci-Col) and two -80°C freezers (Thermo Fisher), a phase-contrast inverted microscope (EVOS, AMG, Life technologies), a fluorescent microscope (EVOS, AMG, Life technologies) and a light microscope (Olympus).
Adjacent to the stem cell research area, we also have access to shared equipment in a 12,640 sq. ft laboratory. The shared equipment includes; two balances (Mettler Toledo AB104, Madell electronic), one biosafety cabinet (Forma Scientific), shaking incubator (New Brunswick, Max Q), cold room (ColPak, Lab Hallway), one ice machine (Hoshizaki America Inc.), fume hood (Forma Scientific), an oven, two centrifuges (Eppendorf 5810R), five -80°C freezers (four Thermo Scientific, one Revco), two -20°C freezers (VWR, Kenmore), a POLAstar Omega Microplate Reader (BMG LABTECH), Odyssey Infrared Imaging System (Li-COR Bioscience), two humidified CO2 incubators (Forma series II, Thermo Electron Cooperation), confocal microscope (Nikon TE2000-U), cell culture inverted microscope (Nikon, TMS), ultra-pure water system (Milli-Q Direct) and one autoclave (Steris, AMSCO Lab250). Assays of CFU-f count, differentiation (Staining & microscopy) will be performed in this HEC lab facility.
The CVM research space located in the HEC also includes a 100 sq. ft computational facility dedicated to the applied genomic center. This room is dedicated to a computational infrastructure including a 30 node parallel cluster computer containing nodes with either an Athlon 1.7 processor and 512 MB ram or dual Pentium III processors and 1GB of ram.
Research space in the Banfield Veterinary Clinic Center, Western University of Health Sciences, Pomona:
The BVCC houses a ~300 sq. ft room reserved the needs of non-terminal research. The room is equipped with: two computers and desks for faculty, visitors, and students working on scholarly writing or data analysis; a refrigerator freezer combo; a vortex; and two computational stations to run immunoassays. This room is also reserved for the storage of equipment used in clinical projects (e.g. pressure mats for gait analysis). This space is assigned to CVM faculty members for other uses, such as ELISA assays, on an as-needed basis.
Research space in Rodney P. Wineberg Center (RWC/URC), Western University of Health Sciences, Pomona:
The laboratory at the RWC encompasses an 8,550 sq. ft research area. This large area is subdivided into different departmental laboratories. The designated CVM laboratory is a 1540 sq. ft laboratory space with a 120 sq. ft. cell-culture room. The laboratory is designed to allow standard molecular assays related to molecular biology, immunology, and gene expression. This laboratory contains a biosafety hood, a chemical hood, two water baths (20L & 6L), two incubators, a CO2 incubator (New Brunswick, Galaxy 170R), a shaker (GeneMate, BioExpress), a microplate reader (Opsy MR, Dynex Technology), a multi-pulse vortexer (Glas-Col), a light microscope with camera attachments, balances, hot plates, gel electrophoresis equipment, nanodrop bioanalyzer (NanoVue), a Biorad PCR Cycler, two refrigerators (Fisher Scientific), three -20°C freezers (Kenmore, Sci-Cool, Fisher Scientific), high speed centrifuge (Sorvall-Discovery 100E), a Beckman ultracentrifuge, a Multi-Pulse vortexer (Clas-Col), a flow cytometer (Cytomics FC500), ice machine, and another liquid nitrogen tank. The 120 sq. ft. cell-culture room is equipped with a CO2 humidified incubator, a biosafety cabinet (Forma Scientific Class II A/B3), a table top refrigerated centrifuge (Thermo-IEC), and a cell-culture microscope (Nikon-TMS).
Adjacent to this CVM lab, shared equipment is accessible in a 3,000 sq. ft laboratory. The shared equipment includes: two biosafety cabinets (Nuaire Class II A/B3 and Thermo Scientific Class II A2); seven microcentrifuges (three Eppendrof model 5417C, two Denville model 210A and two Denville model 260D); two refrigerated microcentrifuges (Eppendrof model 5810R); a spectrophotometer (GE model Nanovue); multiple gel electrophoresis apparatus; a tissue homogenizer (MISONIX); two gel documentation digital imagining stations (BioRad VersaDoc and Fotodyne Inc.); four thermocyclers (three Applied Bisystems model Veriti 96 wells and one BioRad model MJ Mini 64 wells); and one Real-Time PCR (BioRad, model MJ Minid with miniOpticon 64 wells) to be used for quantative RT-PCR assays. Moreover, an adjacent shared equipment laboratory includes a fluorescent microscope, ultracentrifuges, seven -80°C freezers (four Revco, two SANYO, one Thermo Scientific) and a real-time PCR cycler (Applied Biosystem-StepOnePlus). A dishwasher, distilled and ultra-purified water systems, two autoclaves, and an adjacent cold room (4°C) are available for common use.
Other space relevant to CVM research at Western University of Health Sciences, Pomona:
In addition to the facilities above, histology sections can be processed and stained in the pathology laboratory in Veterinary Pathology Center (VPC) located in a building adjacent to BVCC, on the same campus.
Animal facilities are available in the HEC, the RWC/URC, and the Health Professions Center (HPC). Capacities and allocations vary with years but are approximately listed below:
- Animal facilities in the HEC building can house up to 19,200 mice and 240 tanks of zebrafish (number in each tank varies depending on the size each fish)
- Animal facilities in the RWC building can house up to 114 chicken, 1,240 shrews, 8 naked mole rats, and 120 rats.
- Animal facilities in the HPC building can house up to 3,640 mice, 56 guinea pigs, 250 rats, and 120 hamsters.