Hannah Mirrashed, PhD – Research Laboratory Manager
PhD, Carleton University, Ottawa, Canada, 2007
MSc, Carleton University, Ottawa, Canada, 1999
I am a research scientist with more than 5 years of experience in research. Throughout this experience, I was given the opportunity to co-author six peer-reviewed publications and develop skills in a variety of fields, including Molecular Biology, Microbiology, Mycology, Biotherapeutics, organization management and regulation.
I completed a B.Sc. in Microbiology, followed by a M.Sc. and Ph.D. in Molecular Bio/Chemical sciences with a specialization in Biotherapeutics and Pharmacokinetics. During my two post-doctoral positions, I have focused my research on environmental issues such as biological wastewater treatment and risk assessment of microbial contaminants and data generation on microbial consortia stability and Ecotoxicology.
During my B.Sc. in Microbiology, I obtained experience in quality assurance in veterinary sciences and animal pharmacotoxicology by evaluating and analyzing biological and veterinary drugs and vaccines in a Drug Quality Control Laboratory. My graduate work initially focused on the use of large-scale techniques to study fungal populations and their distributions world-wide. I investigated molecular genetic variation in fungal populations (M.Sc.), yeast proteomics and gene expression profiles in response to plant-derived antimicrobial macromolecules. This approach improved bioactive herbal compounds, contributing to the development and optimization of pharmaceutical agents (Pharmacokinetics) (Ph.D).
In addition, prior to my current position, I worked as a research associate in the Food science and Nutrition division at Carleton University from Sep 2011- Feb 2012. My research focused on nutrigenomics and interdisciplinary studies of novel antimicrobial bioactive compounds from natural products. The research focuses on the mode of action of two major antioxidants and anti-inflammatory compounds; (1) Alkylresorcinols (phenolic acids) in cereal bran and (2) melatonin in sour cherries, in food and biological membranes in vitro. The results improved our understanding of the relationship between these natural compounds and their function in living microorganisms.
SKILLS AND EXPERTISE
- Genetic techniques: Microarray, Gene Deletion Array (GDA).
- Molecular biology techniques: QPCR /PCR amplification, Total DNA/RNA extraction, Cloning of PCR products for sequencing, Western blot, Southern blot, Fluorescent in-situ Hybridization (FISH), Northern blot, RFLP/ AFLP, Protein isolation and separation methods, Proteomic.
- Cell culture (Mammalian, yeast and bacterial, transfection, mutation).
- Gel electrophoresis (agarose/acrylamide), DGGE & TGGE.
- RNA synthesis from Yeast DNA and Arabidopsis plasmid.
- Histology and Immunohistochemistry (Paraffin sections).
- Microscopy (Fluorescent & Light Microcopy).
- Experience in quality assurance and quality control related to veterinary drugs, Phytochemistry (herbal remedies and traditional medicines) and environmental bioproducts.
- Experience in the critical evaluation of safety, efficacy data and health risk assessments related to natural health product, microbial hazards and environmental risk assessments of substances (organisms).
- Development of two different softwares. Yeast Features (YF) and Growth Detector (GD) at Carleton University and University of Waterloo. These softwares are designed to assist in the mode of action studies of medicinal compounds.
- Extensive experience in risk assessment of microbial contaminants and prepare assessment reports with respect to both human and environmental health hazards.
- Skilled and ability in planning and conducting scientific research for the characterization of microbial consortia and bioproducts and preparation of a comprehensive report on standard operating procedures (SOP).
- Excellent background in drafting, review and critical evaluation of scientific and other reports identify and analyze multidisciplinary data and provide advice to the Scientific Authority in the form of a scientific and regulatory report and recommendations.
- Knowledge of current and emerging issues, trends and challenges related to toxicology or biotechnology.
José Santiago Aguilar, PhD-Research Technician
PhD, Universidad Complutense, Madrid, 1981.
BSc, Universidad Complutense, Madrid, 1977.
I have extensive research experience in Molecular and Cell Biology. My research has resulted in the publication of over fifty papers in peer-reviewed journals. Before joining WesternU, I have worked in different research institutions in the USA (Northwestern, U. Illinois at Chicago, U. California Irvine) and abroad (Instuto Cajal, Madrid; Instituto Eduardo DeRobertis, Buenos Aires; Bath University, UK). During this research work, I have trained many graduate and undergraduate students publishing their work in peer-review journals.
My research work has focused mainly on cell membrane receptor interactions and signaling. I have carried out studied on different membrane G protein coupled receptors (cholinergic muscarinic, adenosine). A hallmark of these studies was the development of a novel system to generate proteo-liposomes and utilize it to study receptor interactions with membrane proteins. I have also studied ionotropic receptors (cholinergic nicotinic, GABA). A significant conclusion of these studies was the demonstration that allosteric modulators affect both the rate of activation and desensitization of receptors by agonists.
In addition, I have done research on the interactions of enveloped viruses (herpes simplex) with the cell membrane during penetration and cell-to-cell passage. In this work, I did research on gene expression (viral and cellular) during infection. Important accomplishments related to this work were the development of micro-arrays to study herpes infection and the demonstration that polysaccharides are involved in cell-to-cell passage of herpes virus.
I have combined my research work with teaching activities. I have taught Basic Virology and Molecular Biology lab in UCI, Nutritional Biochemistry in CSULB, I have collaborated in post-graduate courses in Northwestern University, the University of Illinois at Chicago and University of Buenos Aires (Argentina) and I have been an invited professor in the University of La Laguna (Spain).
In my research and teaching work I have utilized a broad variety of methodological procedures including, molecular biology, biochemistry, electrophysiology and fluorescence dynamic analysis in life cells and animals, as detailed below.
Research Skills and Expertise
- Molecular Biology : Micro array procedures (fluorescent and colloidal metal procedures); Southern and Northern blots; PCR (qualitative and quantitative); Preparation of total RNA and polyA RNA from cells and tissues /Gradient fractionation of poly A RNA ; In vitro synthesis of mRNA/ Preparation and labeling of RNA probes; Library screening and isolation of clones/Subcloning of DNA in plasmids; Sequencing; DNA Mutations; Transfection of cells / Preparation of stable transfected cells.
- Biochemistry: Quantification of proteins; Column chromatography of proteins (Gel filtration, ion-exchange, affinity); Gel electrophoresis of proteins; Preparation of subcellular fractions; Extraction of lipids and proteolipids / Thin layer chromatography of lipids; Enzymatic assays; Radioligand binding assays.
- Immunology: Antibodies generation; ELISA, Western blots; Immunohistochemical procedures.
- Cell Biology and Virology: Tissue culture (different cell lines); Preparation of virus stocks; Quantization of virus by plaque assay; Histochemistry of cells and tissue slices.
- Microscopy: Confocal; Two-photon; Intravital.
- Electrophysiology: Microinjection of RNA to frog oocytes; Voltage-clamp recording in frog oocytes.
- Animal handling: Rats, mice, rabbits, frog, insects; Drug and pathogens injections (intracranial and other routes); Surgery.
- Computer: Microsoft Office (Word, Excel, Power Point); Graphical Statistical Analysis (Prism); Molecular Biology tools; Clampfit (Electrophysiology);SimFCS (Fluorescence dynamics).
Jonathan A Alvarez, MS
MS, Western University of Health Sciences, Pomona, CA, 2013
BA, University of Redlands, Redlands, CA, 2009
I am a Research Associate with 2 years of post-graduate laboratory experience. In those two years I have had the opportunity to work and develop skills in the fields of Structural Biology, Molecular Biology, Chemistry, Cellular Biology, Biochemistry, and Protein Chemistry. I also have experience teaching in primary and secondary public education system.
I received my BA in Biology from the University of Redlands, with two minors in Chemistry and Environmental Studies. During my undergraduate degree I worked for a low-income clinic helping to teach Diabetes Health courses to the local community. After receiving my BA I taught Life Science, Biology, and Earth Science to high school grades 9-12.
While studying for my MS in Biomedical Science I joined a Structural Biology lab where I focused my research utilizing Crystallography to determine unknown protein structures. Here, I also gained experience in Molecular Biology and Biochemistry.
After receiving my Masters I worked as a Lab Manager and Research Assistant for the Graduate College of Biomedical Sciences at WesternU. There, I managed multiple projects and was responsible to train several graduate students and volunteers. My research focused on protein expression, protein purification, and crystallography. My work there in that lab played a key role in the awarding of a 5-year, multi-million dollar R01-NIH Grant.
My current research focuses on Cell and Cancer Biology. Here I am managing multiple projects using multiple cancer cells lines, induced pluripotent stem cells, and human embryonic stem cells. Thus far, we have made exciting breakthroughs in cell differentiation and efforts to re-sensitize drug-resistant cancer cells.
Research Skills and Expertise
- Molecular biology techniques: QPCR /PCR amplification, DNA extraction, Cloning of PCR products for sequencing
- Cell culture: Mammalian and bacterial, hESC and iPSC, transfection
- Gel electrophoresis (agarose/acrylamide): SDS-PAGE, Western Blot, Cross-linking Assays, Non-Denaturing Gels
- Microscopy: Fluorescent and Light Microcopy
- Automated Liquid Handling: High-throughput robotics (Rigaku)
- Biochemistry: Quantification of proteins; Column chromatography of proteins (Gel filtration, affinity); Gel electrophoresis of proteins
- Bioinformatics: BLAST and ExPASy
- Computer: Microsoft Office (Word, Excel, Power Point); Adobe (Photoshop and Illustrator); Modeling (Phyre, PyMol, Dali); Crystallographic (COOT, CCP4i, HKL2000, Blu-Ice); Linux and Windows OS