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College of Veterinary Medicine

CVM Research Technical Support

Hannah Mirrashed, PhD – Research Laboratory Manager

PhD, Carleton University, Ottawa, Canada, 2007
MSc, Carleton University, Ottawa, Canada, 1999

hmirrashed@weternu.edu

I am a research scientist with more than 5 years of experience in research. Throughout this experience, I was given the opportunity to co-author six peer-reviewed publications and develop skills in a variety of fields, including Molecular Biology, Microbiology, Mycology, Biotherapeutics, organization management and regulation. I completed a B.Sc. in Microbiology, followed by a M.Sc. and Ph.D. in Molecular Bio/Chemical sciences with a specialization in Biotherapeutics and Pharmacokinetics.  During my two post-doctoral positions, I have focused my research on environmental issues such as biological wastewater treatment and risk assessment of microbial contaminants and data generation on microbial consortia stability and Ecotoxicology. During my B.Sc. in Microbiology, I obtained experience in quality assurance in veterinary sciences and animal pharmacotoxicology by evaluating and analyzing biological and veterinary drugs and vaccines in a Drug Quality Control Laboratory.  My graduate work initially focused on the use of large-scale techniques to study fungal populations and their distributions world-wide. I investigated molecular genetic variation in fungal populations (M.Sc.), yeast proteomics and gene expression profiles in response to plant-derived antimicrobial macromolecules. This approach improved bioactive herbal compounds, contributing to the development and optimization of pharmaceutical agents (Pharmacokinetics) (Ph.D). In addition, prior to my current position, I worked as a research associate in the Food science and Nutrition division at Carleton University from Sep 2011- Feb 2012. My research focused on nutrigenomics and interdisciplinary studies of novel antimicrobial bioactive compounds from natural products.  The research focuses on the mode of action of two major antioxidants and anti-inflammatory compounds; (1) Alkylresorcinols (phenolic acids) in cereal bran and (2) melatonin in sour cherries, in food and biological membranes in vitro.   The results improved our understanding of the relationship between these natural compounds and their function in living microorganisms.

Research Skills and Expertise

  • Genetic techniques:  Microarray, Gene Deletion Array (GDA).
  • Molecular biology techniques: QPCR /PCR amplification, Total DNA/RNA extraction, Cloning of PCR products for sequencing, Western blot, Southern blot, Fluorescent in-situ Hybridization (FISH), Northern blot, RFLP/ AFLP, Protein isolation and separation methods, Proteomic.
  • Cell culture (Mammalian, yeast and bacterial, transfection, mutation).
  • Gel electrophoresis (agarose/acrylamide), DGGE & TGGE.
  • RNA synthesis from Yeast DNA and Arabidopsis plasmid.
  • Histology and Immunohistochemistry (Paraffin sections).
  • Microscopy (Fluorescent & Light Microcopy).
  • Experience in quality assurance and quality control related to veterinary drugs, Phytochemistry (herbal remedies and traditional medicines) and environmental bioproducts.
  • Experience in the critical evaluation of safety, efficacy data and health risk assessments related to natural health product, microbial hazards and environmental risk assessments of substances (organisms).
  • Development of two different softwares. Yeast Features (YF) and Growth Detector (GD) at Carleton University and University of Waterloo. These softwares are designed to assist in the mode of action studies of medicinal compounds.
  •  Extensive experience in risk assessment of microbial contaminants and prepare assessment reports with respect to both human and environmental health hazards.
  • Skilled and ability in planning and conducting scientific research for the characterization of microbial consortia and bioproducts and preparation of a comprehensive report on standard operating procedures (SOP).
  •  Excellent background in drafting, review and critical evaluation of scientific and other reports identify and analyze multidisciplinary data and provide advice to the Scientific Authority in the form of a scientific and regulatory report and recommendations.
  •  Knowledge of current and emerging issues, trends and challenges related to toxicology or biotechnology.

 

José Santiago Aguilar, PhD-Research Technician

PhD, Universidad Complutense, Madrid, 1981.
BSc, Universidad Complutense, Madrid, 1977.

jsaguilar@westernu.edu

I have extensive research experience in Molecular and Cell Biology. My research has resulted in the publication of over fifty papers in peer-reviewed journals.  Before joining WesternU, I have worked in different research institutions in the USA (Northwestern, U. Illinois at Chicago, U. California Irvine) and abroad (Instuto Cajal, Madrid; Instituto Eduardo DeRobertis, Buenos Aires; Bath University, UK).   During this research work, I have trained many graduate and undergraduate students publishing their work in peer-review journals. My research work has focused mainly on cell membrane receptor interactions and signaling. I have carried out studied on different membrane G protein coupled receptors (cholinergic muscarinic, adenosine). A hallmark of these studies was the development of a novel system to generate proteo-liposomes and utilize it to study receptor interactions with membrane proteins.  I have also studied ionotropic receptors (cholinergic nicotinic, GABA). A significant conclusion of these studies was the demonstration that allosteric modulators affect both the rate of activation and desensitization of receptors by agonists. In addition, I have done research on the interactions of enveloped viruses (herpes simplex) with the cell membrane during penetration and cell-to-cell passage. In this work, I did research on gene expression (viral and cellular) during infection.  Important accomplishments related to this work were the development of micro-arrays to study herpes infection and the demonstration that polysaccharides are involved in cell-to-cell passage of herpes virus. I have combined my research work with teaching activities. I have taught Basic Virology and Molecular Biology lab in UCI, Nutritional Biochemistry in CSULB, I have collaborated in post-graduate courses in Northwestern University, the University of Illinois at Chicago and University of Buenos Aires (Argentina) and I have been an invited professor in the University of La Laguna (Spain). In my research and teaching work I have utilized a broad variety of methodological procedures including, molecular biology, biochemistry, electrophysiology and fluorescence dynamic analysis in life cells and animals, as detailed below.

Research Skills and Expertise

  • Molecular Biology Micro array procedures (fluorescent and colloidal metal procedures); Southern and Northern  blots;  PCR (qualitative and quantitative); Preparation of total RNA and polyA RNA from cells and tissues /Gradient fractionation of  poly A RNA ; In vitro synthesis of mRNA/ Preparation and labeling of RNA probes; Library screening and isolation of clones/Subcloning of DNA in plasmids; Sequencing; DNA Mutations; Transfection of cells / Preparation of stable transfected cells.
  • Biochemistry:  Quantification of proteins; Column chromatography of proteins (Gel filtration, ion-exchange, affinity); Gel electrophoresis of proteins; Preparation of subcellular fractions; Extraction of lipids and proteolipids / Thin layer chromatography of lipids; Enzymatic assays; Radioligand binding assays.
  • Immunology:  Antibodies generation; ELISA, Western blots;  Immunohistochemical procedures.
  • Cell Biology and Virology: Tissue culture (different cell lines); Preparation of virus stocks; Quantization of virus by plaque assay; Histochemistry of cells and tissue slices.
  • Microscopy:  Confocal; Two-photon; Intravital.
  • Electrophysiology: Microinjection of RNA to frog oocytes; Voltage-clamp recording in frog oocytes.
  • Animal handling: Rats, mice, rabbits, frog, insects; Drug and pathogens injections (intracranial and other routes); Surgery.
  • Computer: Microsoft Office (Word, Excel, Power Point); Graphical Statistical Analysis (Prism); Molecular Biology tools; Clampfit (Electrophysiology);SimFCS (Fluorescence dynamics).

 

 

CHEN XIE

PhD, The Chinese University of Hong Kong, Hong Kong, 2010
MS, China Pharmaceutical University, China, 2004
BS, China Pharmaceutical University, China, 2001

cxie@westernu.edu

Dr. Xie Chen

I have extensive research experience in cellular and molecular biology, analytical methods and many in vitro and in vivo models in pharmaceutical sciences. During these years, I have published total 13 research papers, 9 conference abstracts, 1 patient and 2 book chapters. I received my B.Sc and M.Sc degree in Biopharmaceutics and Ph.D degree in Biomedical Sciences with a specialization in cancer research. I have been a visiting scholar at Metastasis and Angiogenesis Research Group in Cardiff University in 2009 and Advancement of Drug Research and Evaluation Center in Western University of Health Sciences in 2011, respectively. I ever worked as a project manager at the Research and Development Division in a pharmaceutical company in China after I obtained my master degree. During that time, my major responsibility was to research and develop new anti-cancer drugs from batch work to clinic trials as well as to register new and generic anti-cancer drug to China Food and Drug administration (CFDA). After obtaining Ph.D degree in Hong Kong, I have worked for the School of Pharmacy in the Chinese University of Hong Kong as research associate for two years. My project focused on a bio-activity guided in vitro pharmacokinetic method to improve the quality control of Chinese Medicine. I also obtained experience to develop bioanalytical assays to determine the concentration of the major ingredients of Chinese herbs in human/rat plasma. Prior to my current position, I worked as a post-doctoral fellow at school of pharmacy in Western University of Health Sciences from 2015-2016. My research focused on development of an orally effective fusion protein for Hemophilia patients. In addition to the lab work, I gained teaching experience for the graduate course titled Research Techniques: Theory and Practice with the topics of Separation Methods-HPLC and LC-MS at the College of Pharmacy in Western University of Health Sciences.

Research Skills and Expertise

  • Molecular/Cell Biology
    Cell Culture, primary cell preparation and cell transfection
    DNA/RNA extraction
    RT-PCR and real-time PCR
    Flow Cytometry
    Immunofluorescent and Immunohistochemistry staining technology
    ELISA
    Cancer cell function studies about cell proliferation, apoptosis, adhesion, motility, migration (would healing), invasion and soft agar assay.
    Western blot analysis
    Tumor xenograft experiment in nude mice
    Ultrasound microbubble-mediated gene transfer technology
    Short circuit current technology for ion transport measurement
    Intracellular pH measurement; extracelluar pH measurement
    Confocal Imaging

Pharmaceutical sciences

  • Extraction and isolation of traditional Chinese medicine
    Analytical methods: HPLC-DAD, HPLC-MS/MS, GC, UV, IR, TLC
    In vitro model to test drug permeability and transport
    Pharmacokinetic study using animal models
    Expression and purification protein
    Isolation, purification and bioanalytical applications for macromolecules polysaccharide