College of Veterinary Medicine

CVM Research Facilities

Description of Research Facilities

The laboratory spaces allocated to the College of Veterinary Medicine (CVM) are located in three buildings within walking distance of each on the campus at Western University of Health Sciences in Pomona, California: the Health Education Center (HEC), Veterinary Clinic Center (VCC) on the east side, and the Rodney P. Wineberg Center, formerly the University Research Center, (RWC/URC) on the west side of the campus.

Research space in the Health Education Center, Western University of Health Sciences, Pomona:

The CVM research space in the Health Education Center (HEC) comprises a newly remodeled 200 ft2 laboratory strictly dedicated to the isolation and culture of stem cells.  Adjacent to the stem cell room, we have access to shared equipment in a 12,640 ft2 laboratory.  The CVM research space located in the HEC center also includes 100 ft2 dedicated to the applied genomic center with: a 30 node parallel cluster computer containing multiple nodes with either an Athlon 1.7 processor and 512 MB ram or dual Pentium III processors and 1GB of ram; residing in a dedicated computational facility providing appropriate temperature control.


CVM clean room (1,540 ft2) equipment:

• Three (3) CO2 and O2 humidified incubators (New Brunswick)
• One (1) CO2 humidified incubator (New Brunswick)
• Three (3) Biosafety cabinet (Logica, LabCONCO)
• Table top refrigerated centrifuge (Eppendrof Model 5810R)
• 5L analog water bath
• Analytical balance
• Shaking incubator
• Liquid nitrogen tank dedicated to stem cell banking (Thermo Scientific)
• Vacuum aspirator collection system
• 38CF glass refrigerator
• One (1) -20 °C and two (2)-80 °C freezers
• phase-contrast inverted microscope (EVOS, AMG, Life technologies)
• Fluorescent microscope (EVOS, AMG, Life technologies) and a light microscope (Olympus)
• QPCR (CFX- Bio-Rad)
• NanoDrop Lite (Thermo Fisher)
• Oven (HERATHERM, Thermo Scientific)
• Freeze-Dry Vacuum & Pump- (FreeZoon, 2.5, LabCONCO)
• Neon Transfection System (Life Technology)
• One (1) Flow Cytometer (BD Accuri, C6)
• a pH meter (Fisher Scientific)
• Hybridization chamber (Isotemp, Fisher Scientific)
• Trans blot® SD semi dry transfer (Bio rad)

WesternU main lab (12,640 ft2) shared equipment:

• Two (2) balances (Mettler Toledo AB104, Madell electronic)
• Biosafety cabinet (Forma Scientific)
• Shaking Incubator (New Brunswick, Max Q)
• Cold room (ColPak, Lab Hallway)
• Ice machine (Hoshizaki America Inc.)
• Fume hood (Forma Scientific)
• Oven
• Two (2) centrifuges (Eppendorf 5810R)
• Five (5) -80 °C freezers (four Thermo Scientific, one Fisher Scientific )
• Two (2) -20°C freezers (VWR, Kenmore)
• Two humidified CO2 incubators (Forma series II, Thermo Electron Cooperation)
• Confocal microscope (Nikon TE2000-U)
• Cell culture inverted microscope (Nikon, TMS)
• Ultra-pure water system (Milli-Q Direct)
• Autoclave (Steris, AMSCO Lab250)
• One (1) POLAstar Omega Microplate Reader (BMG LABTECH
• Odyssey Infrared Imaging System (Li- COR Bioscience)
• Four (4) Chemical storage cabinets

Research space in the WesternU Pet Health Center, Western University of Health Sciences, Pomona:

The WesternU Pet Health Center (PHC) houses a 300 ft2 room serving the needs of non- terminal animal research. This room stores equipment used in clinical projects (such as pressure mats for gait analysis) and is equipped with two computers and desks for faculty, visitors and students working on scholarly writing or data analysis. The room is also equipped with a refrigerator/freezer combo, a vortex and two computational stations to run immunoassays.

Research space in Rodney P. Wineberg Center (RWC/URC), Western University of Health Sciences, Pomona:

The Rodney Wineberg Center for Research (RWC) comprises an 8,550 ft2 shared research area. This large area is subdivided into different departmental laboratories. The designated CVM laboratory is a 1,540 ft2 laboratory space with two cell culture rooms (120-130 ft2). The laboratory is designed to allow standard molecular assays related to molecular biology, immunology and gene expression. Adjacent to the CVM lab, shared equipment is accessible in a 3,000 sq. foot laboratory shared by the entire campus.


CVM main lab (1,540 ft2) equipment:

• Biosafety hood (CBS Scientific Co.)
• Chemical hood (Hanson)
• Water bath 20L (Fisher Scientific)
• Bead bath 5L (Fisher Scientific)
• Incubators (Fisher Scientific)
• Light microscope with camera attachments (Nikon Eclipse Ni)
• Balances (Ohaus, Mettler Toledo)
• Hot plates (Fisher Scientific)
• Gel electrophoresis equipment (Fisher Scientific)
• Nanodrop (NanoVue)
• Two thermocyclers (Eppendorf, Bio-Rad)
• Three (3) refrigerators (Fisher Scientific, Kenmore, Traulsen)
• Six (6) -20O freezers (Kenmore, Sci- Cool, Fisher Scientific)
• High speed centrifuge (Sorvall-Discovery 100E)
• Ultracentrifuge (Beckman)
• Ice machine (Manitowoc)
• Liquid nitrogen tank
• Inverted light microscope (Nikon)
• Two (2) incubators (New Brunswick, Fisher Scientific)
• a CO2 incubator (Fisher Scientific), a shaker (New Brunswick)
• a Microplate reader (Cambrex)
• a multi-pulse vortexer (Glas-Col)
• Two (2) UV transilluminators (UVP, Fisher Scientific)
• Two (2) ovens (Shel Lab, Memmert)
• Ultrasonic cleaner (VWR)
• pH meter (Fisher Scientific)
• multilabel plate reader (PerkinElmer)
• two Spectrophotometers (Beckman, Jenway)
• Spectrofluorophotometer (Shimadzu)
• Screen eraser (Bio-Rad)
• Plate washer (Biotrak II)

WesternU main lab (3,000 ft2) shared equipment:

• Two (2) biosafety cabinets (Nuaire Class II A/B3 and Thermo Scientific Class II A2)
• Ten (10) microcentrifuges (three Eppendrof model 5417C and one model 5418, two Denville model 210A, and two Denville model 260D, one Fisher-Scientific accuspin 17R, Spectrafuge)
• Two (2) refrigerated microcentrifuges (Eppendrof model 5810R)
• Spectrophotometer (GE model Nanovue)
• Multiple gel electrophoresis apparatus
• Tissue homogenizer (MISONIX)
• Two (2) gel documentation digital imagining stations (Bio-Rad VersaDoc, Fotodyne Inc.)
• Four (4) Thermocyclers (three Applied Biosystems and two Bio-Rad)
• Real-Time PCR (Bio-Rad, model MJ Minid with miniOpticon 64 wells) to beused for quantative RT-PCR assays
• Vortex mixer (Labnet)
• pH meter (Oakton)
• Three (3) -20º C freezers (two Summit, Kenmore)
• Two (2) platform rocker (Labnet, Fisher)
• Fraction collector (Bio-Rad)
• Balances

CVM cell culture room (120 ft2) equipment:

• CO2 humidified incubator (Thermo)
• Biosafety cabinet (Forma Scientific Class II A/B3)
• Table top refrigerated centrifuge (Thermo-IEC)
• Cell culture microscope (Nikon-TMS)
• -20º C freezer (Sanyo)
• 10 L water bath (Fisher Scientific)
• Rocker platform (Elmeco)
• Inverted microscope (Fisher Scientific)
• CO2 incubator (Fisher Scientific)

WesternU auxiliary lab equipment:

• Fluorescent microscope
• Ultracentrifuges
• Seven (7) -80°C freezers (four Revco,one SANYO, one Thermo Scientific, one Panasonic)
• Real-time PCR cycler (Applied Biosystem-StepOnePlus)
• Dishwasher
• Distilled and Ultra Water purifying systems (Barnstead)
• Two (2) autoclaves (Sterilmatic)
• An adjacent cold room (4°C)

CVM cell culture room (130 ft2) equipment:

• Biosafety cabinet (Nuaire)
• Bead bath 10L (Fisher Scientific)
• Refrigerator (Kenmore)
• CO2 incubator (Fisher Scientific)
• Microcentrifuge

Other space relevant to CVM research at Western University of Health Sciences, Pomona:

In addition to the facilities above, histology sections can be processed and stained in the pathology laboratory in Veterinary Pathology Center (VPC) located in a building adjacent to VCC, on the same campus.