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College of Veterinary Medicine

CVM Research Facilities

Description of Research Facilities

The laboratory spaces allocated to the College of Veterinary Medicine (CVM) are located in three buildings within walking distance of each on the campus at Western University of Health Sciences in Pomona, California: the Health Education Center (HEC), Veterinary Clinic Center (VCC) on the east side, and the Rodney P. Wineberg Center, formerly the University Research Center, (RWC/URC) on the west side of the campus.

Research space in the Health Education Center, Western University of Health Sciences, Pomona:


Equipment
The CVM research space in the Health Education Center (HEC) comprises a newly remodeled 200 ft2 laboratory strictly dedicated to the isolation and culture of stem cells.  Adjacent to the stem cell room, we have access to shared equipment in a 12,640 ft2 laboratory.  The CVM research space located in the HEC center also includes 100 ft2 dedicated to the applied genomic center with: a 30 node parallel cluster computer containing multiple nodes with either an Athlon 1.7 processor and 512 MB ram or dual Pentium III processors and 1GB of ram; residing in a dedicated computational facility providing appropriate temperature control.

CVM Main Lab (1,540 ft2) equipment:

• Three (3) -20c Freezers (Fisher Scientific)
• 5L Analog Water Bath (Fisher Scientific, IsoTemp 205)
• Two (2) -80C Freezer (Fisher Scientific, Panasonic)
• Two (2) Analytical Balances (Mettler Toledo, DENVER)
• Beads Bath (Fisher Scientific)
• Three (3) BioSafety Cabinets (Logica, LabConco)
• Three (3) CO2 and O2 Humidified Incubators (New Brunswick, Galaxy 170R)
• CO2 Humidfied Incubator (New Brunswick, Galaxy 170R)
• Three (3) Combo Fridge-Freezer (Kenmore, Thermo Scientific)
• Two (2) Dry Block Heaters (Major Scientific, Fisher Scientific)
• Two (2) Electrophoresis Chambers (Bio-Rad)
• Flow Cytometer (BD Accuri, C6)
• Fluorescent Microscope (EVOS, AMG, Life Technologies)
• Freeze-Dry Vacuum and Pump (FreeZoon 2.5, LabConco)
• Three (3) Hot-plate Stirrers (Fisher Scientific)
• Hybridization Chamber (IsoTemp, Fisher Scientific)
• Light Microscope (Olympus)
• Three (3) Liquid Nitrogen Tanks [dedicated to stem cell banking] (Thermo Scientific)
• NanoDrop Lite (Thermo Fisher)
• Neon Transfection System (Life Technology)
• Oven (Thermo Scientific)
• pH Meter (Fisher Scientific)
• Phase Contrast Inverted Microscope (EVOS, AMG, Life Technologies)
• Plateshaker (Benchmark)
• Two (2) Thermal Cycler PCR (Eppendorf; AB Applied Biosystems)
• RealTime PCR (CFX, Bio-Rad)
• Refrigerated Microcentrifuge (Labnet)
• Refrigerator (VWR)
• RT Basic Magnetic Stirrer (Thermo Fisher)
• Shaking Incubator (New Brunswick Scientific)
• Sonicator (VWR model 50T)
• Sliding Door Fridge (Fisher Scientific)
• Tabletop Refrigerated High Speed Centrifuge (Eppendorf Model 5810R)
• High Speed Centrifuge (Eppendorf Model 5702)
• Transblot SD Semi Dry Transfer (Bio-Rad)
• Three (3) Vacuum Aspirator Collection Systems (Thermo Fisher)
• Four (4) Vortex Mixers (Scientific Industrial, Fisher Scientific, Benchmark)

WesternU Main Lab (12,640 ft2) Shared Equipment:

• -80C Freezer (Thermo Scientific)
• Acid and Corrosive Storage Cabinet (Justrite)
• Autoclave (Steris, AMSCO Lab250)
• Two (2) Balances (Mettler Toledo AB104, Madell Electronic)
• Biosafety Cabinet (Forma Scientific)
• Capillary Western Blot Instrument (ProteinSimple)
• Cell Culture Inverted Microscope (Nikon, TMS)
• Four (4) Chemical Storage Cabinets (Justrite)
• ColdRoom (ColPak, Lab Hallway)
• Two (2) Confocal Microscopes (ZEISS, LSM 880, LSM T-PMT, Nikon TE2000U)
• Seven (7) Flammable Liquid Storage Cabinets (Justrite)
• Three (3) Fume Hoods (Forma Scientific, Thermo Fisher, Hamilton Scientific)
• Two (2) Humidified CO2 Incubators (Forma Series II, Thermo Electron Corp)
• Ice Machine (Hoshizaki American Inc.)
• Two (2) Liquid Nitrogen Storage Tanks (Taylor-Wharton)
• Odyssey Infrared Imaging System (Li-COR Bioscience)
• POLAstar Omega Microplate Reader (BMG LABTECH)
• Shaking Incubator (New Brunswick, Max Q)
• Two (2) Tabletop Refrigerated Centrifuges (Eppendorf 5810R)
• Ultra-pure Water System (Milli-Q Direct)

Research space in the WesternU Pet Health Center, Western University of Health Sciences, Pomona:

The WesternU Pet Health Center (PHC) houses a 300 ft2 room serving the needs of non- terminal animal research. This room stores equipment used in clinical projects (such as pressure mats for gait analysis) and is equipped with two computers and desks for faculty, visitors and students working on scholarly writing or data analysis. The room is also equipped with a refrigerator/freezer combo, a vortex and two computational stations to run immunoassays.

Research space in Rodney P. Wineberg Center (RWC/URC), Western University of Health Sciences, Pomona:


Equipment
The Rodney Wineberg Center for Research (RWC) comprises an 8,550 ft2 shared research area. This large area is subdivided into different departmental laboratories. The designated CVM laboratory is a 1,540 ft2 laboratory space with two cell culture rooms (120-130 ft2). The laboratory is designed to allow standard molecular assays related to molecular biology, immunology and gene expression. Adjacent to the CVM lab, shared equipment is accessible in a 3,000 sq. foot laboratory shared by the entire campus.

CVM main lab (1,540 ft2) equipment:

• Agilent Bioanalyzer (Agilent)
• AKTA Pure L25 [Protein Purification] (GE Healthcare BioSciences Corp)
• Anaerobic Incubator
• Anaerobic Workstation (Whitley DG250, Microbiology International)
• Two (2) Balances (Ohaus, Mettler Toledo)
• 5L Bead Bath (Fisher Scientific)
• Biosafety Hood (CBS Scientific Co.)
• Chemical Hood (Hanson)
• CO2 Incubator w/ Shaker (New Brunswick)
• Dry Bath System 
• Eight (8) -20C Freezers (Kenmore, Sci-Cool, Fisher Scientific)
• Four (4) Gel Electrophoresis (Fisher Scientific)
• Hemogenizing Kit (Omni TIP)
• High-Speed Centrifuge (Sorvall-Discovery 100E)
• Two (2) Hot Plates (Fisher Scientific)
• Five (5) Incubators (3 Fisher Scientific; SHEL Lab; Memmert)
• Inverted Light Microscope (Nikon)
• Li-Cor c-DiGit Blot Scanner
• Light Microscope w/ Camera Attachments (Nikon Eclipse Ni)
• Two (2) Liquid Nitrogen Tanks
• Microplate Reader (Cambrex)
• Multilabel Plate Reader (PerkinElmer)
• Multi-Pulse Vortexer (Glas-Col)
• Nanodrop (NanoVue)
• Two (2) pH Meters (Fisher Scientific)
• Six (6) Refrigerators (Fisher Scientific, Kenmore, Traulsen)
• SPD2010A220 Speed Vac (Thermo Scientific)
• Spectrofluorphotometer (RF-5301PC-Shimadzu)
• Spectrophotometer (Beckman)
• Two (2) Thermocyclers (Eppendorf; Bio-Rad)
• Ultracentrifuge (Beckman)
• Ultrasonic Cleaner (VWR)
• Vitl Life Plate Sealers
• 20L Water Bath (Fisher Scientific)

WesternU main lab (3,000 ft2) shared equipment:

• Three (3) -20C Freezers (2 Summit; 1 Kenmore)
• Two (2) Balances (Metler)
• Two (2) Biosafety Cabinets (Nuaire Class II A/B3; Thermo Scientific Class II A2)
• Fraction Collector (Bio-Rad)
• Two (2) Gel Documentation Digital Imagining Stations (Bio-Rad Versa Doc; Fotodyne Inc)
• Ice Machine (Manitowoc)
• Ten (10) Microcentrifuges (3 Eppendorf; 2 Denville; Fisher Scientific; Spectrafuge)
• Multiple Gel Electrophoresis Apparatus (Fisher Scientific)
• pH Meter (Oakton)
• Plate Washer (Biotrak II)
• Two (2) Platform Rockers (Labnet; Fisher)
• Real-Time PCR [to be used for quantitative RT-PCR assays] (Bio-Rad)
• Two (2) Refrigerated Microcentrifuges (Eppendorf Model 5810R)
• Screen Eraser (Bio-Rad)
• Spectrophotometer (GE Model Nanovue)
• Five (5) Thermocyclers (3 Applied Biosystems; 2 Bio-Rad)
• Tissue Homogenizer (MISONIX)
• Ultracentrifuge (Beckman)
• Vortex Mixer (Labnet) 


CVM Cell Culture Room B104A (110ft2):

• Three (3) 10L Water Bath (Fisher Scientific
• -20C Freezer (Sanyo)
• Analytical Balance (Mettler-Toledo)
• Balance (Scout Professional)
• Biosafety Cabinet (Forma Scientific Class II A/B3)
• Cell Culture Microscope (Nikon TMS)
• CO2 Humidified Incubator (VWR)
• CO2 Incubator (Fisher Scientific)
• Refrigerator (Sanyo)
• Three (3) Hot Plate Magnetic Stirrers (Fisher Scientific)
• Inverted Microscope (Fisher Scientific)
• Magentic Stirrer (Benchmark)
• Two (2) Microfuges (Thermo Fisher)
• NanoDrop (Thermo Fisher)
• Two (2) PCR Cycles (Applied Biosystems)
• pH Meter (Fisher Scientific)
• Plate Reader (Cambrex)
• Rocker Platform (Elmeco)
• Sonicator (VWR)
• Tabletop Refrigerated Centrifuge (Thermo IEC)
• Vortex Mixer (Fisher Scientific)
• Vortexer (Labnet)

CVM Cell Culture Room B103C1 (120ft2):

• -20C Freezer (Kenmore)
• 10L Bead Bath (Isotemp)
• Benchtop Microcentrifuge (Denville 210A)
• Biosafety Cabinet (Nuaire Class II Type A1B3)
• CO2 Incubator (Panasonic)
• Hotplate (Benchmark)
• Light Inverted Microscope (Motic)
• Tabletop Refrigerated Centrifuge (Eppendorf Model 5810R)
• Vortex (Fisher Scientific)

CVM Cell Culture Room B103C (118ft2):

• 10L Bead Bath (Isotemp)
• Biosafety Cabinet (Thermo Forma Class II A2)
• Two (2) CO2 Incubators (Fisher Scientific)
• Light Inverted Microscope (Nikon)
• Tabletop Refrigerated Centrifuge (Eppendorf Model 5810R)

CVM Cell Culture Room B103A (130ft2):

• 10L Bead Bath (Fisher Scientific)
• Biosfety Cabinet (Nuaire)
• CO2 Incubator (Fisher Scientific)
• Refrigerator (GE)
• Tabletop Refrigerated Centrifuge (Eppendorf Model 5810R)

WesternU auxiliary lab equipment:

• Seven (7) -80C Freezers (4 Revco; 1 Sanyo; 1 Thermo Scientific; 1 Panasonic)
• Adjacent Cold Room
• Two (2) Autoclaves (Sterilmatic)
• Distilled and Ultra Water Purifying Systems (Barnstead)
• Fluorescent Microscope (Nikon)
• Real-Time PCR Cycler (Applied Biosystem StepOne Plus)

Other space relevant to CVM research at Western University of Health Sciences, Pomona:

In addition to the facilities above, histology sections can be processed and stained in the pathology laboratory in Veterinary Pathology Center (VPC) located in a building adjacent to VCC, on the same campus.